Cyclin E2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29686
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Between 354 and 404
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Cyclin E2 Antibody - BSA Free (NBP3-29686) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Cyclin E2 Antibody - BSA Free
Immunoprecipitation:Cyclin E2 AntibodyNBP3-29686] -
Immunoprecipitation:Cyclin E2 AntibodyNBP3-29686] -Detection of human CCNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNE2 antibody NBP3-29686 (lot NBP3-29686-1) used for IP at 6 µg per reaction. CCNE2 was also immunoprecipitated by rabbit anti-CCNE2 antibody. For blotting immunoprecipitated CCNE2, NBP3-29686 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for Cyclin E2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate pH 7 -8.
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cyclin E2
Cyclin E2 (CCNE2) is an approximately 45-50 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression and the initiation of DNA replication. Cyclin E2 is required for G1/S phase progression and cell cycle reentry from G0 phase. Cyclin E2 most highly expressed in testis, thymus, and brain and is frequently upregulated in cancer. Cyclin E2 contains two cyclin box folds (aa 141-231 and aa 241-361). An alternate splice form lacks 45 amino acids within the first cyclin box fold.
Alternate Names
CCNE2, CYCE2
Gene Symbol
CCNE2
Additional Cyclin E2 Products
Product Documents for Cyclin E2 Antibody - BSA Free
Product Specific Notices for Cyclin E2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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