Cyclin D1 Antibody (SPM587) [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34816H

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-32840

Alexa Fluor 350

NBP2-34816AF350

Alexa Fluor 405

NBP2-34816AF405

Alexa Fluor 488

NBP2-34816AF488

Alexa Fluor 532

NBP2-34816AF532

Alexa Fluor 594

NBP2-34816AF594

Alexa Fluor 647

NBP2-34816AF647

Alexa Fluor 700

NBP2-34816AF700

Alexa Fluor 750

NBP2-34816AF750

Alexa Fluor Plus 405

NBP2-34816AFP405

Alexa Fluor Plus 488

NBP2-34816AFP488

Alexa Fluor Plus 555

NBP2-34816AFP555

Alexa Fluor Plus 594

NBP2-34816AFP594

Alexa Fluor Plus 647

NBP2-34816AFP647

Alexa Fluor Plus 680

NBP2-34816AFP680

Allophycocyanin

NBP2-34816APC

Biotin

NBP2-34816B

CoraFluor 1

NBP2-34816CL1

DyLight 350

NBP2-34816UV

DyLight 405

NBP2-34816V

DyLight 488

NBP2-34816G

DyLight 550

NBP2-34816R

DyLight 594

NBP2-34816DL594

DyLight 650

NBP2-34816C

DyLight 680

NBP2-34816FR

DyLight 755

NBP2-34816IR

FITC

NBP2-34816F

Janelia Fluor 525

NBP2-34816JF525

Janelia Fluor 549

NBP2-34816JF549

Janelia Fluor 646

NBP2-34816JF646

Janelia Fluor 669

NBP2-34816JF669

mFluor Violet 450 SE

NBP2-34816MFV450

mFluor Violet 500 SE

NBP2-34816MFV500

mFluor Violet 610 SE

NBP2-34816MFV610

PE

NBP2-34816PE

PerCP

NBP2-34816PCP

Forumulation

Catalog #

Azide and BSA Free

NBP2-34816

IHC-Prediluted

NBP2-44585

Product Specifications
Applications
Spectra Viewer
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

HRP

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SPM587

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin D1 Antibodies »

Product Specifications

Immunogen

Human recombinant full length cyclin D1 protein (Uniprot: P24385)

Localization

Nuclear

Specificity

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Marker

G1-Cyclin & Mantle Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for Cyclin D1 Antibody (SPM587) [HRP]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Recommended applications are based on validated applications from the unconjugated base product NBP2-34816. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cyclin D1

During the cell cycle of most somatic cells, DNA synthesis (S-phase) and mitosis (M-phase) are separated by two gap phases (G1 and G2) of varying duration. Thus, a typical eukaryotic cell sequentially passes through G1, S, G2, and M and back into G1 during a single cycle.12 Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins.13 These proteins are the regulatory subunits of the cyclin dependent kinases (CDKs), which are responsible for the phosphorylation of several cellular targets. Complexes of cyclins and CDKs play a key role in cell cycle control. Within the complexes, the cyclin subunit serves a regulatory role, whereas CDKs have a catalytic protein kinase activity.14 Members of the cyclin family of proteins combine with a CDK subunit to form the active kinase, which initiates G2 to M and G1 to S transition. The latter are controlled by cyclins termed G1 cyclins, which commit the cell to DNA replication. Therefore, the cell cycle can be considered as a cyclin cycle which is controlled by biochemical modifications and formation of complex(es) with CDKs.15 At least five candidate G1-phase cyclins, termed cyclins C, D1 , D2 , D3 , and E have been identified in mammalian cells. Each of these cyclins can associate with one or more of the CDK family members. D-type cyclins are induced during the G1 phase of the mammalian cell cycle in response to a variety of mitogenic growth factors. The three distinct members of the D-type cyclin family are differentially and combinatorially xpressed in various cell lineages. Once induced, the D-type cyclins accumulate in complexes with CDKs, whose kinase activity is thought to be necessary for driving cells into S phase. The major catalytic partners of the D-type cyclins are CDK4 and CDK6, but at least some D-type cyclins also interat with other CDKs, including CDK2 and CDK5. Cyclin D1- and D2-associated CDK4 and/or CDK6 kinase activities have been detected in mid-G1, prior to the activation of any other known CDK, and they culminate in late G1 phase. The cyclin D3-associated CDK4 and/or CDK6 exhibit kinase activities at the G1/S transition. Cyclins D1 , D2 and D3 can be distinguished by their slightly different mobilities on denaturing gels. Under these conditions, the apparent masses are 36, 33-35 and 31-34 kD for cyclins D1 , D2 and D3 , respectively. Because D-type cyclins probably serve as integrators of growth factor-induced signals with the cell cycle clock, aberrant expression of these proteins might play a role in disrupting the normal timing of events governing G1 progression and, in so doing, contribute to oncogenesis. Indeed, a link between tumor formation and inappropriate expression of cyclins has been established.16,17 Overexpression of this protein as a result of chromosomal rearrangement, occurs in parathyroid tumors and centrocytic lymphomas, and amplification of the cyclin D1 gene has been observed in a significant percentage of other cancers, including breast, squamous, and esophageal arcinomas. Immunochemical techniques provide a convenient and sensitive method for detection of these cyclins in human tumor tissues. Such assays facilitate studies directed toward correlating the phenotypic subtypes and aggressiveness of particular human tumors known to exhibit cyclin D1 overexpression and enable studies with other types in which cyclins D2 and D3 are similarly implicated in pathogenesis. The availability of monoclonal antibody reacting specifically with cyclin D1 enables the subcellular detection and localization of cyclin D1 and the measurement of relative differences in cyclin D1 levels as a function of cell cycle phase.

Alternate Names

Bcl-1, CCND1, Cyl-1, PRAD1

Gene Symbol

CCND1

UniProt

P25322 (Mouse), P39948 (Rat)

Additional Cyclin D1 Products

Product Documents for Cyclin D1 Antibody (SPM587) [HRP]

Product Datasheets

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COA

SDS

Product Specific Notices for Cyclin D1 Antibody (SPM587) [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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