Cullin 3 Antibody (7W3E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15821
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7W3E3 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 669-768 of human Cullin 3 (Q13618). LHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Cullin 3 Antibody (7W3E3) (NBP3-15821) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Cullin 3 Antibody (7W3E3)
![Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821]](https://resources.bio-techne.com/images/products/Cullin-3-Antibody-7W3E3-Western-Blot-NBP3-15821-img0004.jpg "Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821]")
Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821]
Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] -
Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded rat brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] -
Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded mouse brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for Cullin 3 Antibody (7W3E3)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CUL3
Long Name
Cullin 3
Alternate Names
KIAA0617, NEDAUS, PHA2E
Gene Symbol
CUL3
Additional CUL3 Products
Product Documents for Cullin 3 Antibody (7W3E3)
Product Specific Notices for Cullin 3 Antibody (7W3E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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