The immunogen recognized by this antibody maps to a region between residue 1025 and the C-terminus (residue 1071) of human Chromosome Region Maintenance 1 (Exportin 1) (NP_003391.1).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:33188200).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit CRM1 Antibody - BSA Free (NB100-79802) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. Anti-CRM1 Antibody: Cited in 16 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CRM1 Antibody - BSA Free

Western Blot: CRM1 Antibody [NB100-79802]

Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802]

CRM1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-79802-img0028.jpg

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802]

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: CRM1 Antibody [NB100-79802]

Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. NB100-79802 used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP. CRM1 was also less efficiently immunoprecipitated by NB100-79811, which recognize upstream epitopes on CRM1.

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802]

CRM1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-79802-img0027.jpg

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802]

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Simple Western: CRM1 Antibody [NB100-79802]

Simple Western: CRM1 Antibody [NB100-79802] - Simple Western lane view shows a specific band for CRM1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] -

A – Fluorescence images of cells transfected with thepTurboGFP-N-PON2 plasmid and then stained with anti-CRM1 antibodies. B– Fluorescence images of cells transfected with plasmidsencoding different fragments of PON2 (1–27 a.a.; 1–83 a.a.;1–168 a.a.) or GFP alone as a control

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] -

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] - Effect of TSA treatment on the subcellular localization of Crm1.Control ES or Nup98-HoxA9 expressing ES cells were cultured in the presence or absence of 50 nM TSA for 24 hr. Then, the cells were fixed & stained with antibodies against FLAG (M2) & Crm1. Merged images of FLAG (green) & Crm1 (red) are shown. Nuclei were stained with DAPI. Bar, 10 μm. DAPI, 4',6-diamidino-2-phenylindole; ES, embryonic stem; TSA, trichostatin A.DOI:http://dx.doi.org/10.7554/eLife.09540.020 Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/09540), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] -

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] - A – Fluorescence images of cells transfected with thepTurboGFP-N-PON2 plasmid & then stained with anti-CRM1 antibodies. B– Fluorescence images of cells transfected with plasmidsencoding different fragments of PON2 (1–27 a.a.; 1–83 a.a.;1–168 a.a.) or GFP alone as a control Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30397533), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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Applications for CRM1 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 to 1:10000

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-10 ug/mg lysate

Simple Western

1:500

Western Blot

1:2000-1:10000

Application Notes

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:500, apparent MW was 110 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CRM1

CRM1 mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1. Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

Long Name

Chromosome Region Maintenence 1 Homolog

Alternate Names

CRM-1, Exp1, Exportin 1, XPO1

Entrez Gene IDs

7514 (Human); 103573 (Mouse)

Gene Symbol

XPO1

UniProt

O14980 (Human)

Additional CRM1 Products

Product Documents for CRM1 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CRM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CRM1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79802

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all CRM1 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Theoretical MW

Description

Scientific Data Images for CRM1 Antibody - BSA Free

Western Blot: CRM1 Antibody [NB100-79802]

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802]

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802]

Western Blot: CRM1 Antibody [NB100-79802]

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802]

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802]

Simple Western: CRM1 Antibody [NB100-79802]

Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] -

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] -

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] -

Applications for CRM1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Immunoprecipitation

Simple Western

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CRM1

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CRM1 Products

Product Documents for CRM1 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CRM1 Antibody - BSA Free