CrkL Antibody (JE50-21)

Western Blot: CrkL Antibody (JE50-21) [NBP3-32222]

Western Blot: CrkL Antibody (JE50-21) [NBP3-32222] - Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (NBP3-32222) at 1/1,000 dilution.

Lane 1: K-562 cell lysate (20 ug/Lane)
Lane 2: A431 cell lysate (20 ug/Lane)
Lane 3: HT-29 cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: NIH/3T3 cell lysate (20 ug/Lane)
Lane 6: C6 cell lysate (20 ug/Lane)
Lane 7: Mouse brain tissue lysate (40 ug/Lane)
Lane 8: Rat brain tissue lysate (40 ug/Lane)

Predicted band size: 34 kDa
Observed band size: 37 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32222) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CrkL Antibody (JE50-21) [NBP3-32222]

Immunohistochemistry: CrkL Antibody (JE50-21) [NBP3-32222] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CrkL antibody (NBP3-32222) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32222) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: CrkL Antibody (JE50-21) [NBP3-32222]

Flow Cytometry: CrkL Antibody (JE50-21) [NBP3-32222] - Flow cytometric analysis of A431 cells labeling CrkL.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32222, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).