Crk [p Tyr221] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23252
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Chicken, Xenopus
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phospho-Crk (Tyr-221) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-221 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, and has 40% homology with the conserved site in CrkL (Tyr-207). The site is not found in Crk I. [UniProt# P46108]
Modification
p Tyr221
Specificity
The antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Crk [p Tyr221] Antibody
![Crk [p Tyr221] Antibody](https://resources.bio-techne.com/images/products/nbp3-23252_rabbit-crk-p-tyr221-pab-19920231252363.jpg "Western Blot: Crk [p Tyr221] Antibody [NBP3-23252] -")
Western Blot: Crk [p Tyr221] Antibody [NBP3-23252] -
Western Blot: Crk [p Tyr221] Antibody [NBP3-23252] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Crk (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk (C-terminal region) NBP3-23251 (lanes 1 & 2) and Crk (Tyr-221) phospho-specific NBP3-23252 (lanes 3 & 4).Applications for Crk [p Tyr221] Antibody
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Crk
Crk belongs to a family of adaptor molecules, which link extracellular growth and differentiation stimuli with formation of signal transduction protein complexes. Crk is mainly composed of Src homology 2 (SH2) and Src homology 3 (SH3) domains, which serve as docking sites for signalling proteins, including various receptors, cytoplasmic kinases, and GTPase regulators. These signal transduction pathways lead to modulation of cell adhesion, cell migration and immune cell responses.
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog
Alternate Names
adapter molecule crk, avian sarcoma virus CT10 (v-crk) oncogene homolog, CRKII, p38, Proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene homolog (avian)
Gene Symbol
CRK
Additional Crk Products
Product Documents for Crk [p Tyr221] Antibody
Product Specific Notices for Crk [p Tyr221] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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