Crk Antibody (4Y2C2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16830
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4Y2C2 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Crk (P46108). MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPAL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Crk Antibody (4Y2C2) (NBP3-16830) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Crk Antibody (4Y2C2)
![Western Blot: Crk Antibody (4Y2C2) [NBP3-16830]](https://resources.bio-techne.com/images/products/Crk-Antibody-4Y2C2-Western-Blot-NBP3-16830-img0001.jpg "Western Blot: Crk Antibody (4Y2C2) [NBP3-16830]")
Western Blot: Crk Antibody (4Y2C2) [NBP3-16830]
Western Blot: Crk Antibody (4Y2C2) [NBP3-16830] - Western blot analysis of extracts of various cell lines, using CRK/Crk Rabbit mAb (NBP3-16830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Applications for Crk Antibody (4Y2C2)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Crk
Crk belongs to a family of adaptor molecules, which link extracellular growth and differentiation stimuli with formation of signal transduction protein complexes. Crk is mainly composed of Src homology 2 (SH2) and Src homology 3 (SH3) domains, which serve as docking sites for signalling proteins, including various receptors, cytoplasmic kinases, and GTPase regulators. These signal transduction pathways lead to modulation of cell adhesion, cell migration and immune cell responses.
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog
Alternate Names
adapter molecule crk, avian sarcoma virus CT10 (v-crk) oncogene homolog, CRKII, p38, Proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene homolog (avian)
Gene Symbol
CRK
Additional Crk Products
Product Documents for Crk Antibody (4Y2C2)
Product Specific Notices for Crk Antibody (4Y2C2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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