CREB Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90364

Novus Biologicals, part of Bio-Techne
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NBP1-90364
NBP1-90364-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CREB Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: STIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CREB Antibody - BSA Free (NBP1-90364) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-CREB Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CREB Antibody - BSA Free

CREB Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: CREB Antibody - BSA Free [NBP1-90364]

Immunocytochemistry/ Immunofluorescence: CREB Antibody - BSA Free [NBP1-90364]

Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: CREB Antibody [NBP1-90364]

Immunohistochemistry-Paraffin: CREB Antibody [NBP1-90364]

Immunohistochemistry-Paraffin: CREB Antibody [NBP1-90364] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
CREB Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: CREB Antibody - BSA Free [NBP1-90364]

Chromatin Immunoprecipitation-exo-Seq: CREB Antibody - BSA Free [NBP1-90364]

ChIP-Exo-Seq composite graph for Anti-CREB1 (NBP1-90364) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
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Applications for CREB Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in Human foreskin cell lysate, human epidermis lysate, separated by Size, antibody dilution of 1:50

Reviewed Applications

Read 3 reviews rated 4.7 using NBP1-90364 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CREB

CREB (cAMP responsive element binding protein 1) stimulates transcription. CREB is involved in learning and memory. This protein is known to have interactions with HIST1H3B, HIST1h3A, HIST1H3C, HIST1H3D and HIST1H3E. CREB has been studied in relation to several diseases and disorders including Rubinstein-Taybi syndrome, Alzheimer's disease, leukemia and brain disease.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Gene Symbol

CREB1

Additional CREB Products

Product Documents for CREB Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CREB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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