Collagen XI alpha 2 Antibody (473) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43728
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (93%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 473
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Collagen XI alpha 2. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
172 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Collagen XI alpha 2 Antibody (473) - Azide and BSA Free (NBP2-43728) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Collagen XI alpha 2 Antibody (473) - Azide and BSA Free
![Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728]](https://resources.bio-techne.com/images/products/Collagen-XI-alpha-2-Antibody-473-Western-Blot-NBP2-43728-img0002.jpg "Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728]")
Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728]
Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1) 12 % SDS-PAGE COL11A2 antibody [473] dilution: 1:5000
Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] -
Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL11A2 antibody [GT473] (NBP2-43728) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Collagen XI alpha 2 Antibody (473) [NBP2-43728] -
COL11A2 antibody [GT473] detects COL11A2 protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL11A2 protein stained by COL11A2 antibody [GT473] (NBP2-43728) diluted at 1:500.
Blue: Hoechst 33342 staining.
Applications for Collagen XI alpha 2 Antibody (473) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Collagen XI alpha 2
Alternate Names
collagen alpha-2(XI) chain, collagen, type XI, alpha 2, DFNA13, DFNB53, HKE5, PARP, pro-a2 chain of collagen type XI, STL3
Gene Symbol
COL11A2
Additional Collagen XI alpha 2 Products
Product Documents for Collagen XI alpha 2 Antibody (473) - Azide and BSA Free
Product Specific Notices for Collagen XI alpha 2 Antibody (473) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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