Collagen VI Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-6583
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Feline
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Collagen VI from human and bovine placenta (Uniprot: P12109)
Reactivity Notes
Feline reactivity reported in scientific literature (PMID: 33091431).
Localization
Extracellular matrix.
Specificity
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen VI alpha 1 and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
Scientific Data Images for Collagen VI Antibody [Biotin]
Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody [Biotin] [NB120-6583] - Collagen VI Antibody [Biotin] [NB120-6583] - collagen VI antibody 1:400 45 min RT showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.
Applications for Collagen VI Antibody [Biotin]
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:100
Western Blot
1:5000-1:10000
Application Notes
This product is suitable for western blot, immunoprecipitation, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen VI
Gene Symbol
COL6A1
UniProt
Additional Collagen VI Products
Product Documents for Collagen VI Antibody [Biotin]
Product Specific Notices for Collagen VI Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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