Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08487
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]](https://resources.bio-techne.com/images/products/Collagen-IV-Antibody-rCOL4-4742-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08487-img0001.jpg "Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # rCOL4/4742
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified human placental extract
Localization
Secreted. Extracellular matrix.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07596).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487] - Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV Recombinant Mouse Monoclonal Antibody (rCOL4/4742)![Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]](https://resources.bio-techne.com/images/products/Collagen-IV-Antibody-rCOL4-4742-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08487-img0002.jpg "Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]")
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487] - Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV Recombinant Mouse Monoclonal Antibody (rCOL4/4742).Applications for Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Collagen IV
Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.
Alternate Names
BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
Gene Symbol
COL4A1
Additional Collagen IV Products
Product Documents for Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free
Product Specific Notices for Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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