Collagen II Antibody (2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05169
![Immunohistochemistry-Paraffin: Collagen II Antibody (2) - BSA Free [NBP1-05169]](https://resources.bio-techne.com/images/products/Procollagen-Type-2A-Antibody-003-02-Immunohistochemistry-Paraffin-NBP1-05169-img0006.jpg "Immunohistochemistry-Paraffin: Collagen II Antibody (2) - BSA Free [NBP1-05169]")
![Immunohistochemistry-Paraffin: Collagen II Antibody (2) - BSA Free [NBP1-05169]](https://resources.bio-techne.com/images/products/Procollagen-Type-2A-Antibody-003-02-Immunohistochemistry-Paraffin-NBP1-05169-img0005.jpg "Immunohistochemistry-Paraffin: Collagen II Antibody (2) - BSA Free [NBP1-05169]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant expressed PIIANP
Epitope
Epitope is located at the amino-terminal part of procollagen type IIA
Specificity
Procollagen type IIA N-terminal propeptide
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Concentration for any given batch will be between 0.85 - 1.15 mg/mL.
Scientific Data Images for Collagen II Antibody (2) - BSA Free
Immunohistochemistry-Paraffin: Collagen II Antibody (2) - BSA Free [NBP1-05169]
Immunohistochemistry-Paraffin: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with NBP1-05169 developed with peroxidase-labeled goat anti-mouse Ig (blue color).![Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]](https://resources.bio-techne.com/images/products/Procollagen-Type-2A-Antibody-003-02-Immunohistochemistry-NBP1-05169-img0003.jpg "Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]")
Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]
Immunohistochemistry: Collagen II Antibody (003-02) - BSA Free [NBP1-05169] - Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Positive stain of Canine Chondrocytes incubated overnight at room temperature at 1:100 dilution. Image courtesy of Dragana Ponjevic, Dr. Matyas Laboratory, University of Calgary.![Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]](https://resources.bio-techne.com/images/products/Procollagen-Type-2A-Antibody-003-02-Immunohistochemistry-NBP1-05169-img0004.jpg "Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]")
Immunohistochemistry: Collagen II Antibody (2) - BSA Free [NBP1-05169]
Immunohistochemistry: Procollagen Type 2A Antibody (003-02) [NBP1-05169] - Human chondrocytes stained with NBP1-05169 overnight at room temperature, 1:100 dilution. Image courtesy of Dragana Ponjevic, Dr. Matyas Laboratory, University of Calgary.Applications for Collagen II Antibody (2) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:1000
Application Notes
ELISA: NBP1-05169 also reacts with recombinant PIIANP coated directly onto the microtiter well. IHC: NBP1-05169 diluted in 10%FCS/TBS is well suited for immunohistochemistry on formalin-fixed, paraffin-embedded fetal tissue. Use in Canine reported by customer review.
Reviewed Applications
Read 1 review rated 5 using NBP1-05169 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Collagen II
Alternate Names
Chondrocalcin, COL2A1, SEDC
Gene Symbol
COL2A1
Additional Collagen II Products
Product Documents for Collagen II Antibody (2) - BSA Free
Product Specific Notices for Collagen II Antibody (2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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