Cofilin [p Ser3] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05394

Novus Biologicals, part of Bio-Techne
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NBP3-05394-20ul
NBP3-05394-100ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CFL1 Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S3 of human Cofilin (NP_005498.1). MASGV

Modification

p Ser3

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Cofilin [p Ser3] Antibody - BSA Free (NBP3-05394) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cofilin [p Ser3] Antibody - BSA Free

Western Blot: Cofilin [p Ser3] AntibodyAzide and BSA Free [NBP3-05394]

Western Blot: Cofilin [p Ser3] AntibodyAzide and BSA Free [NBP3-05394]

Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: Cofilin [p Ser3] Antibody - Azide and BSA Free [NBP3-05394]

Immunocytochemistry/ Immunofluorescence: Cofilin [p Ser3] Antibody - Azide and BSA Free [NBP3-05394]

Immunocytochemistry/Immunofluorescence: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunofluorescence analysis of methanol-fixed HeLa cells using Cofilin antibody (NBP3-05394).
Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody - Azide and BSA Free [NBP3-05394]

Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody - Azide and BSA Free [NBP3-05394]

Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Cofilin antibody (NBP3-05394).

Applications for Cofilin [p Ser3] Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CFL1

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Long Name

cofilin 1

Alternate Names

CFL, cofilin, cofilin-1, HEL-S-15

Gene Symbol

CFL1

Additional CFL1 Products

Product Documents for Cofilin [p Ser3] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cofilin [p Ser3] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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