CDC73/HRPT2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB200-184
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Parafibromin using the numbering given in TrEMBL entry Q6P1J9 (GeneID 79577).
Specificity
NB200-184 is specific for human Parafibromin protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CDC73/HRPT2 Antibody - BSA Free (NB200-184) is a polyclonal antibody validated for use in WB and IP. Anti-CDC73/HRPT2 Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CDC73/HRPT2 Antibody - BSA Free
Western Blot: CDC73/HRPT2 Antibody [NB200-184]
Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184]
Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184] - Detection of human CDC73/HRPT2 (Parafibromin) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC73/HRPT2 antibody NB200-184 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated CDC73/HRPT2, NB200-184 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for CDC73/HRPT2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-4 ug/mg lysate
Western Blot
1:5000-1:20000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CDC73
Long Name
Cell Division Cycle 73
Alternate Names
HPT-JT, HRPT2, Parafibromin
Entrez Gene IDs
79577 (Human)
Gene Symbol
CDC73
UniProt
Additional CDC73 Products
Product Documents for CDC73/HRPT2 Antibody - BSA Free
Product Specific Notices for CDC73/HRPT2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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