Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 336445

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC25A Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CDC25A
Glu2-Leu523
Accession # AAA58415

Specificity

Detects human, mouse, and rat CDC25A in direct ELISAs and Western blots. In direct ELISA and Western blots, this antibody does not cross-react with recombinant human (rh) CDC25B or rhCDC25C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for CDC25A Antibody (336445) [Dylight 405]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CDC25A

The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase.

Long Name

Cell Division Cycle 25A

Alternate Names

CDC25A2, CDC25A2-CAG isoform, cell division cycle 25 homolog A (S. cerevisiae), cell division cycle 25 homolog A (S. pombe), cell division cycle 25A, Dual specificity phosphatase Cdc25A, EC 3.1.3.48, M-phase inducer phosphatase 1

Gene Symbol

CDC25A

Additional CDC25A Products

Product Documents for CDC25A Antibody (336445) [Dylight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDC25A Antibody (336445) [Dylight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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