Mouse CD96 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB5690A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 630612
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD96
Val22-Met536
Accession # Q3U0X8
Val22-Met536
Accession # Q3U0X8
Specificity
Detects mouse CD96 in direct ELISAs. In direct ELISAs, no
cross-reactivity with recombinant human CD96v2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD96 APC-conjugated Antibody
Detection of CD96 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD96 APC-conjugated Monoclonal Antibody (Catalog # FAB5690A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD96 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD96
Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily. It is expressed on CD4+ and CD8+ T cells, NK and NKT cells, resting monocytes and gamma delta T cells. Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355). Unlike human, there is no splice variant in the second V-type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437-602. Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.
Alternate Names
CD96, TACTILE
Gene Symbol
CD96
UniProt
Additional CD96 Products
Product Documents for Mouse CD96 APC-conjugated Antibody
Product Specific Notices for Mouse CD96 APC-conjugated Antibody
For research use only
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