CD9 Antibody (IVA50) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-494
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Cited:
Human, Bovine
Applications
Validated:
Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2 Clone # IVA50
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Bovine thrombocytes.
Specificity
The antibody IVA50 reacts with CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse CD9 Antibody (IVA50) - BSA Free (NB500-494) is a monoclonal antibody validated for use in WB, Flow and IP. Anti-CD9 Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD9 Antibody (IVA50) - BSA Free
Immunoprecipitation: CD9 Antibody (IVA50) - BSA Free [NB500-494]
Immunoprecipitation: CD9 Antibody (IVA50) [NB500-494] - Fig. 1. Immunoprecipitation of human CD9 from the biotin-labeled human platelets lysates. Lane 1: immunoprecipitation with anti-CD9 (IVA50) Lane 2: immunoprecipitation with anti-human CD9 control antibody Lane 3: immunoprecipitation with negative control.Flow Cytometry: CD9 Antibody (IVA50) - BSA Free [NB500-494]
Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed) in Analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) purified antibody (concentration in sample 10 ug/ml) GAM APC.Western Blot: CD9 Antibody (IVA50) - BSA Free [NB500-494] -
(a) Mean fluorescence (log scaled) of mEVs under confocal microscopy loaded with Calcein-AM post-lyophilization after removal of exogenous dye via Sepharose G50 spin column. N = 6 images, error bars are representative of standard deviation within each group; biological duplicates. No signal was observed in HEPES control, 10mM TH, and 50mM sucrose groups (Images in Figure S6). Multiple T tests with Bonferroni Correction ( alpha = 0.05), reveals significant differences between fresh and 25mM TH | 75mM TH and between 25 mM TH and 50mM TH | 50mM TH + W. N = 6 images, 3 ROIs each (b) CTDR: DAPI fluorescent ratios (log-scaled) of CTDR loaded fresh mEVs supplemented with TH (50mM) and TH (50mM) + Tryptophan (W; 100 uM) on MDCK cells and 50mM TH + 100 μm Tryptophan mEVs loaded with CTDR uptake post-lyophilization over time in room temperature storage, biological triplicates with N = 10 pictures per sample, outliers removed. (d) Western blotting of lyophilized mEV isolate for CD9, TSG-101, and negative expression of Calnexin. Lyophilized samples were stored at RT for 1 month Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39806456), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for CD9 Antibody (IVA50) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunoprecipitation
1:50
Western Blot
1 ug/ml
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD9
Alternate Names
CD9, DRAP-27, MIC3, p24, TSPAN29
Gene Symbol
CD9
Additional CD9 Products
Product Documents for CD9 Antibody (IVA50) - BSA Free
Product Specific Notices for CD9 Antibody (IVA50) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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