CD81 Antibody (SN206-01)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67722
Recombinant Monoclonal Antibody
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SN206-01 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human CD81 aa 161-210 / 236. (SwissProt: P60033 Human; SwissProt: P35762 Mouse; SwissProt: Q62745 Rat)
Localization
Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CD81 Antibody (SN206-01)
Western Blot: CD81 Antibody (SN206-01) [NBP2-67722]
Western Blot: CD81 Antibody (SN206-01) [NBP2-67722] - Analysis of TAPA1/CD81 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: PC-12 cell lysateLane 2: JAR cell lysateImmunocytochemistry/ Immunofluorescence: CD81 Antibody (SN206-01) [NBP2-67722]
Immunocytochemistry/Immunofluorescence: CD81 Antibody (SN206-01) [NBP2-67722] - Staining CD81 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: CD81 Antibody (SN206-01) [NBP2-67722]
Immunohistochemistry-Paraffin: CD81 Antibody (SN206-01) [NBP2-67722] - Analysis of paraffin-embedded mouse testis tissue using anti-CD81 antibody. Counter stained with hematoxylin.Applications for CD81 Antibody (SN206-01)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Reviewed Applications
Read 1 review rated 4 using NBP2-67722 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD81
Alternate Names
CD81, TAPA-1, TSPAN28
Gene Symbol
CD81
Additional CD81 Products
Product Documents for CD81 Antibody (SN206-01)
Product Specific Notices for CD81 Antibody (SN206-01)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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