CD5 Antibody (CL12334) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15133
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]](https://resources.bio-techne.com/images/products/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0005.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]")
.png "Detection of CD5 in Human Mantle Cell Lymphoma via seqIF™ staining on COMET™")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, COMET
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL12334
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from P06127, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for CD5 Antibody (CL12334) - BSA Free
Detection of CD5 in Human Mantle Cell Lymphoma via seqIF™ staining on COMET™
CD5 was detected in immersion fixed paraffin-embedded sections of human mantle cell lymphoma using Mouse Anti-Human CD5 Monoclonal Antibody (Catalog # NBP3-15133) at 1:50 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.![Western Blot: CD5 Antibody (CL12334) [NBP3-15133]](https://resources.bio-techne.com/images/products/CD5-Antibody-CL12334-Western-Blot-NBP3-15133-img0007.jpg "Western Blot: CD5 Antibody (CL12334) [NBP3-15133]")
Western Blot: CD5 Antibody (CL12334) [NBP3-15133]
Western Blot: CD5 Antibody (CL12334) [NBP3-15133] - Analysis in human cell line MOLT-4.![Immunocytochemistry/ Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133]](https://resources.bio-techne.com/images/products/CD5-Antibody-CL12334-Immunocytochemistry-Immunofluorescence-NBP3-15133-img0006.jpg "Immunocytochemistry/ Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133]")
Immunocytochemistry/ Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133]
Immunocytochemistry/Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133] - Staining of A-431 cells using the Anti-CD5 monoclonal antibody, showing localization in plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively.![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]](https://resources.bio-techne.com/images/products/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0001.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]")
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]](https://resources.bio-techne.com/images/products/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0002.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]")
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]](https://resources.bio-techne.com/images/products/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0003.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]")
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]](https://resources.bio-techne.com/images/products/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0004.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133]")
Applications for CD5 Antibody (CL12334) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000- 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD5
Alternate Names
CD5
Gene Symbol
CD5
Additional CD5 Products
Product Documents for CD5 Antibody (CL12334) - BSA Free
Product Specific Notices for CD5 Antibody (CL12334) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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