CD47 Antibody (B6H12.2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31106
![Flow Cytometry: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Flow-Cytometry-NBP2-31106-img0009.jpg "Flow Cytometry: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]")
![Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-31106-img0012.jpg "Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # B6H12.2
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was raised against CD47.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30833751).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD47 Antibody (B6H12.2) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]
Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Western Blot: CD47 Antibody (B6H12.2)Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Western-Blot-NBP2-31106-img0008.jpg "Western Blot: CD47 Antibody (B6H12.2)Azide and BSA Free [NBP2-31106]")
Western Blot: CD47 Antibody (B6H12.2)Azide and BSA Free [NBP2-31106]
Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0010.jpg "Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]")
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human breast cancer tissue stained with CD47 Antibody (B6H12.2). IHC-P image submitted by a verified customer review.
![Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-NBP2-31106-img0007.jpg "Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]")
![Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-31106-img0011.jpg "Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]")
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0003.jpg "Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]")
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0002.jpg "Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]")
![Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]](https://resources.bio-techne.com/images/products/CD47-Antibody-B6H12-2-Azide-Free-Flow-Cell-Surface-NBP2-31106-img0006.jpg "Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]")
Applications for CD47 Antibody (B6H12.2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug/mL
Immunohistochemistry-Paraffin
0.5 ug/mL
Western Blot
1:1000
Application Notes
This antibody is CyTOF ready.
Reviewed Applications
Read 5 reviews rated 4.8 using NBP2-31106 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD47
Additional CD47 Products
Product Documents for CD47 Antibody (B6H12.2) - Azide and BSA Free
Product Specific Notices for CD47 Antibody (B6H12.2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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