CD44 Antibody (OX-50) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1317
![Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]](https://resources.bio-techne.com/images/products/CD44-Antibody-OX-50-Flow-Cytometry-NB600-1317-img0003.jpg "Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OX-50
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Rat T cell blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.
Localization
Type I membrane protein.
Specificity
This recognizes the rat CD44 cell surface antigen, expressed by T cells, B cells, macrophages and thymocytes.
Marker
Cell Membrane Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD44 Antibody (OX-50) - BSA Free
Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]
Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.![Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) - BSA Free [NB600-1317]](https://resources.bio-techne.com/images/products/CD44-Antibody-OX-50-Immunohistochemistry-Paraffin-NB600-1317-img0005.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) - BSA Free [NB600-1317]")
Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) - BSA Free [NB600-1317]
Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) [NB600-1317] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaining. This antibody clone specifically stained the membranes of the cells close to the bases of the crypts/villi which are known to actively migrate towards the tip of villi.![Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]](https://resources.bio-techne.com/images/products/CD44-Antibody-OX-50-Flow-Cytometry-NB600-1317-img0002.jpg "Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]")
Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]
Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.Applications for CD44 Antibody (OX-50) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50 - 1:100
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 31229690)
Immunohistochemistry
1:50 - 1:100
Immunohistochemistry-Frozen
1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
Immunohistochemistry-Paraffin
1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
Western Blot
reported in scientific literature (PMID 8537452)
Application Notes
This antibody is CyTOF ready.
Reviewed Applications
Read 1 review rated 3 using NB600-1317 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
Additional CD44 Products
Product Documents for CD44 Antibody (OX-50) - BSA Free
Product Specific Notices for CD44 Antibody (OX-50) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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