CD40/TNFRSF5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56127
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A recombinant protein containing the short cytoplasmic domain of the human CD40 molecule was used as immunogen.
Reactivity Notes
Cross reacts with Gerbil.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CD40/TNFRSF5 Antibody - BSA Free
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127]
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127] - FFPE tissue section of human tonsil stained for CD40 expression using this antibody at 1:2000. Staining is seen in the dendritic cells. Hematoxylin-eosin counterstain.Western Blot: CD40/TNFRSF5 Antibody - BSA Free [NB100-56127] -
The contribution of oligodendrocytes to the BDEVs pool is significantly upregulated at 72 h after tMCAO. A Western blots of sBDEVs samples from tMCAO and shams (n = 5 per group) blotted for cell-type-specific markers: PLP and CNP1 are used as protein markers for oligodendrocytes (orange frame); synapsin 1 (Syn1) and NCAM as markers for neurons (green); EAAT1 and EAAT2 as protein markers for astrocytes (pink) and P2Y12 and CD40 as markers for microglia/macrophages (blue). TH is a total mouse brain homogenate loaded in parallel for comparison purposes. TS is a representative total protein staining of the nitrocellulose membranes (TSs of all blots used for these analyses are provided in Suppl. Fig. 5). B Dot plots showing the quantifications of the western blot intensities. For the quantification, each band intensity was first referred to the corresponding lanes of the total protein staining. Both markers for oligodendrocytes were found significantly increased upon stroke. Regarding neuronal markers, NCAM was significantly increased while Syn1 only showed a tendency to be elevated. Exact p-values are given in the main text Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35639208), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for CD40/TNFRSF5 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Immunoprecipitation
1:50 - 1:200
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD40/TNFRSF5
Alternate Names
CD40, TNFRSF5
Gene Symbol
CD40
Additional CD40/TNFRSF5 Products
Product Documents for CD40/TNFRSF5 Antibody - BSA Free
Product Specific Notices for CD40/TNFRSF5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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