CD40/TNFRSF5 Antibody (5C3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43416
Clone 5C3 was used by HLDA to establish CD designation.
![Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]](https://resources.bio-techne.com/images/products/CD40-TNFRSF5-Antibody-5C3-Flow-Cytometry-NBP1-43416-img0004.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5C3
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was CD40.
Reactivity Notes
Mouse reactivity reported in scientific literature (Chechneva O et al).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CD40/TNFRSF5 Antibody (5C3) - BSA Free
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the PE conjugate of NBP1-43416. Analysis of human peripheral blood leukocytes using CD40 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.![Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]](https://resources.bio-techne.com/images/products/CD40-TNFRSF5-Antibody-5C3-Flow-Cytometry-NBP1-43416-img0003.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]")
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the FITC conjugate of NBP1-43416. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-Human CD40 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.Applications for CD40/TNFRSF5 Antibody (5C3) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Application Notes
The 5C3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. This antibody may also be used for immunohistochemical staining of frozen tissue sections. 5C3 has also been reported in in vitro functional studies.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD40/TNFRSF5
Alternate Names
CD40, TNFRSF5
Gene Symbol
CD40
Additional CD40/TNFRSF5 Products
Product Documents for CD40/TNFRSF5 Antibody (5C3) - BSA Free
Product Specific Notices for CD40/TNFRSF5 Antibody (5C3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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