CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54392
![Western Blot: CD3 epsilon Antibody (C3e/1308)Azide and BSA Free [NBP2-54392]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-C3e-1308-Azide-and-BSA-Free-Western-Blot-NBP2-54392-img0002.jpg "Western Blot: CD3 epsilon Antibody (C3e/1308)Azide and BSA Free [NBP2-54392]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), COMET, Multiplex Immunoassay, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # C3e/1308
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human CD3 epsilon fragment (aa 23-119) (Uniprot: P07766)
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 33428661).
Localization
Cell surface and Cytoplasmic
Specificity
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53387).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free
Detection of CD3e�in mouse Intestine�via seqIF� staining on COMET�
CD3 epsilon was detected in frozen sections of mouse Intestine�using Mouse�Anti-Human/Mouse CD3 epsilon,�Monoclonal Antibody at 2ug/mL�at 37� Celsius for 4�minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment�Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia�Catalog # TA-999-DHBH).�Tissue was stained using the Alexa Fluor� 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 Celsius for 2 minutes. (Yellow; Lunaphore�Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore�Catalog # DR100).�Specific staining was localized to the membrane. Protocol available in COMET� Panel Builder.![Western Blot: CD3 epsilon Antibody (C3e/1308)Azide and BSA Free [NBP2-54392]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-C3e-1308-Azide-and-BSA-Free-Western-Blot-NBP2-54392-img0006.jpg "Western Blot: CD3 epsilon Antibody (C3e/1308)Azide and BSA Free [NBP2-54392]")
Western Blot: CD3 epsilon Antibody (C3e/1308)Azide and BSA Free [NBP2-54392]
Western Blot: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).![Flow Cytometry: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392]](https://resources.bio-techne.com/images/products/CD3-epsilon-Antibody-C3e-1308-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54392-img0004.jpg "Flow Cytometry: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392]")
Flow Cytometry: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392]
Flow Cytometry: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Western Blot
1 - 2 ug/ml
Application Notes
Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD3 epsilon
Alternate Names
CD3e
Gene Symbol
CD3E
Additional CD3 epsilon Products
Product Documents for CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free
Product Specific Notices for CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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