CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-45129
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # SKII.4
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Mice were immunized with SK-N-S1 human neuroblastoma cells.
Specificity
This antibody binds an epitope in the extracellular region of human CD155/PVR. It also binds two NK cell receptors CD96 and CD226.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Scientific Data Images for CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free
Flow Cytometry: CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free [NBP3-45129] -
Flow Cytometry: CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free [NBP3-45129] - Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-45129 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.
Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free [NBP3-45129] -
Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free [NBP3-45129] - Immunofluorescence analysis of paraformaldehyde-fixed A431 cells on coverslips stained with NBP3-45129 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45129, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.Applications for CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD155/PVR
Long Name
Poliovirus Receptor
Alternate Names
CD155, HVED, Necl-5, PVR, PVS
Gene Symbol
PVR
Additional CD155/PVR Products
Product Documents for CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free
Product Specific Notices for CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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