CD117/c-kit Antibody (C117/8399R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20927
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # C117/8399R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 50-250) of human CD117/c-kit protein (exact sequence is proprietary)
Localization
Cell surface. Cytoplasm.
Specificity
Anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.
Prepared in 10mM PBS WITHOUT BSA & azide.
Antibody without azide - store at -20 to -80C.
Prepared in 10mM PBS WITHOUT BSA & azide.
Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD117/c-kit Antibody (C117/8399R) - Azide and BSA Free
CD117/c-kit Antibody (C117/8399R)
Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit antibody (C117/8399R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for CD117/c-kit Antibody (C117/8399R) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: 2-4ug/ml For coating, order Ab without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD117/c-kit
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Gene Symbol
KIT
Additional CD117/c-kit Products
Product Documents for CD117/c-kit Antibody (C117/8399R) - Azide and BSA Free
Product Specific Notices for CD117/c-kit Antibody (C117/8399R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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