Key Product Details

Species Reactivity

Human, Primate

Applications

Western Blot, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), Neutralization

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # 21418

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CCL5/RANTES Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL5/RANTES

Specificity

Detects human and primate CCL5/RANTES in direct ELISAs and Western blots. When used in combination with the biotinylated anti-human/primate CCL5/RANTES detection antibody (Catalog # BAF278) in sandwich ELISAs, no significant cross-reactivity or interference is observed with recombinant human (rh) ANG, rhCNTF, rh beta-ECGF, rhEGF, mouse (m) EGF, rhEpo, bovine (b) FGF acidic, rhFGF acidic, bFGF basic, rhFGF basic, rhFGF-4, rhG-CSF, rhGM-CSF, recombinant mouse (rm) GM-CSF, rhGRO alpha, rhIFN-gamma, rhIGF-I, rhIGF-II, rhIL-1 alpha, rmIL-1 alpha, rhIL-1 beta, rmIL‑1 beta, rhIL-1ra, recombinant rat (rr) IL-1ra, rhIL-2, rhIL-3, rmIL-3, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL‑10, rhIL-11, rhLIF, rhMCP‑1, rhM-CSF, rhMIP-1 alpha, rmMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rhOSM, human (h) PDGF, pPDGF, rhPDGF-AA, rhPDGF‑AB, rhPDGF-BB, rhSCF, rmSCF, rhSLPI, rhTGF-alpha, pTGF-beta 1, hTGF-beta 1, rhTGF-beta 1, pTGF-beta 2, rhTGF-beta 2, recombinant canine TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-alpha, rmTNF-alpha, or rmTNF-beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for CCL5/RANTES Antibody (21418) [CoraFluor™ 1]

CCL5/RANTES Antibody (21418) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for CCL5/RANTES Antibody (21418) [CoraFluor™ 1]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB678. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CCL5/RANTES

RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5 (1). This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1 (2). CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites (3).

Alternate Names

RANTES, SISd

Gene Symbol

CCL5

Additional CCL5/RANTES Products

Product Documents for CCL5/RANTES Antibody (21418) [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CCL5/RANTES Antibody (21418) [CoraFluor™ 1]

Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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