Caveolin-2 Antibody - BSA Free

Western Blot: Caveolin-2 Antibody [NBP1-31116]

Western Blot: Caveolin-2 Antibody [NBP1-31116] - Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.

Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116]

Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116] - IP samples: A549 whole cell extract A. 30 ug A549 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of caveolin 2 protein by 4 ug Caveolin 2 antibody 15 % SDS-PAGE The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Immunocytochemistry/ Immunofluorescence: Caveolin-2 Antibody [NBP1-31116] -

Caveolin-2 antibody detects Caveolin-2 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caveolin-2 protein stained by Caveolin-2 antibody (NBP1-31116) diluted at 1:500.
Blue: Hoechst 33342 staining.

Western Blot: Caveolin-2 Antibody - BSA Free [NBP1-31116] -

The S100 protein family affects HNSCC metastasis and is regulated by CAV2.A The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 and SCC25 HNSCC cell lines was evaluated by immunoblotting. B The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 cell lines was evaluated by RT–qPCR. C Alterations in the invasive ability of SCC15 and SCC25 cells following siRNA interference of S100 protein family members. The invasive ability of SCC15 and SCC25 cells was detected using Transwell assays. *, **, *** and **** indicate p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Knockdown Validated: Caveolin-2 Antibody - BSA Free [NBP1-31116] -

CAV2 promotes HNSCC metastasis both in vitro and in vivo.Silencing CAV2 in SCC15 and SCC25 cells by transfection with two individual CAV2 shRNAs significantly decreased CAV2 expression, as detected by western blotting (A) and QT-PCR (B). C, D The silencing of CAV2 significantly inhibited the migration and invasion of SCC15 and SCC25 cells, as evaluated using Transwell assays. (Scale bar, 200 μm). E Images of the macroscopic lung tissues of tail vein-injected mice. F The lung metastasis nodules were counted, and the data from the shCAV2 and negative control groups are summarized. ‘****’ indicates ‘p < 0.0001’. G CAV2 IHC staining and H&E staining to assess lung metastasis in tail vein-injected mice. (Scale bars, 200 μm and 100 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Caveolin-2 Antibody - BSA Free [NBP1-31116] -

High expression of CAV2 is associated with the poor prognosis of HNSCC patients.A IHC analysis of CAV2 levels in 211 human HNSCC samples (scale bar, 100 μm or 400 μm). B Kaplan–Meier survival curve showing the correlation of overall survival and disease-free survival with the CAV2 IHC score. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Caveolin-2 Antibody - BSA Free [NBP1-31116] -

CAV2 promotes HNSCC metastasis both in vitro and in vivo.Silencing CAV2 in SCC15 and SCC25 cells by transfection with two individual CAV2 shRNAs significantly decreased CAV2 expression, as detected by western blotting (A) and QT-PCR (B). C, D The silencing of CAV2 significantly inhibited the migration and invasion of SCC15 and SCC25 cells, as evaluated using Transwell assays. (Scale bar, 200 μm). E Images of the macroscopic lung tissues of tail vein-injected mice. F The lung metastasis nodules were counted, and the data from the shCAV2 and negative control groups are summarized. ‘****’ indicates ‘p < 0.0001’. G CAV2 IHC staining and H&E staining to assess lung metastasis in tail vein-injected mice. (Scale bars, 200 μm and 100 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.