Cav1.3 Antibody (S48) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12893
![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]](https://resources.bio-techne.com/images/products/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0005.jpg "Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]")
![Immunocytochemistry/ Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893]](https://resources.bio-techne.com/images/products/Cav1-3-Antibody-S48-Immunocytochemistry-Immunofluorescence-NBP2-12893-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # S48
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 859-875 of rat Cav1.3
Localization
Membrane, Cell Membrane
Specificity
Detects approx 250 kDa. No cross-reactivity against Cav1.2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Cav1.3 Antibody (S48) - BSA Free
Immunocytochemistry/ Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893]
Immunocytochemistry/Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3Monoclonal Antibody (NBP2-12893) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3Antibody (D) Composite.![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]](https://resources.bio-techne.com/images/products/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0006.jpg "Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]")
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (NBP2-12893) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]](https://resources.bio-techne.com/images/products/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0001.jpg "Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]")
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893]
Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - IHC analysis of Cav1.3 tested on human hippocampus lysates. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.Applications for Cav1.3 Antibody (S48) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Western Blot
1:1000
Application Notes
1 ug/ml\L of Cav1.3 Antibody was sufficient for detection of Cav1.3 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cav1.3
Long Name
Voltage-dependent L-type calcium channel subunit alpha-1D
Alternate Names
CACH3, CACN4, CACNA1D, CACNL1A2, CCHL1A2
Gene Symbol
CACNA1D
Additional Cav1.3 Products
Product Documents for Cav1.3 Antibody (S48) - BSA Free
Product Specific Notices for Cav1.3 Antibody (S48) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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