CASC5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2586
![Western Blot: CASC5 Antibody [NB100-2586]](https://resources.bio-techne.com/images/products/CASC5-Antibody-Western-Blot-NB100-2586-img0007.jpg "Western Blot: CASC5 Antibody [NB100-2586]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human Cancer Susceptibility Candidate 5 using the numbering given in entry NP_733468.2
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CASC5 Antibody - BSA Free
![Western Blot: CASC5 Antibody [NB100-2586]](https://resources.bio-techne.com/images/products/CASC5-Antibody-Western-Blot-NB100-2586-img0005.jpg "Western Blot: CASC5 Antibody [NB100-2586]")
Western Blot: CASC5 Antibody [NB100-2586]
Western Blot: CASC5 Antibody [NB100-2586] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CASC5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.![Immunoprecipitation: CASC5 Antibody [NB100-2586]](https://resources.bio-techne.com/images/products/CASC5-Antibody-Immunoprecipitation-NB100-2586-img0006.jpg "Immunoprecipitation: CASC5 Antibody [NB100-2586]")
Immunoprecipitation: CASC5 Antibody [NB100-2586]
Immunoprecipitation: CASC5 Antibody [NB100-2586] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbitanti-CASC5 antibody used for IP at 6 ug per reaction. CASC5 was alsoimmunoprecipitated by a previous lot of this antibodyl. For blotting immunoprecipitated CASC5, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Applications for CASC5 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 29497093).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CASC5
Alternate Names
AF15q14, Blinkin, blinkin, bub-linking kinetochore protein, Bub-linking kinetochore protein, cancer susceptibility candidate 5, Cancer susceptibility candidate gene 5 protein, Cancer/testis antigen 29, CT29KIAA1570, D40, hKNL-1, hSpc105, kinetochore null 1 homolog, Kinetochore-null protein 1, KNL1, KNL1ALL1-fused gene from chromosome 15q14 protein, protein CASC5, Protein D40/AF15q14
Entrez Gene IDs
57082 (Human)
Gene Symbol
KNL1
UniProt
Additional CASC5 Products
Product Documents for CASC5 Antibody - BSA Free
Product Specific Notices for CASC5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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