Cardiac Troponin T Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13391
![Western Blot: Cardiac Troponin T Antibody [NBP3-13391]](https://resources.bio-techne.com/images/products/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13391-img0004.jpg "Western Blot: Cardiac Troponin T Antibody [NBP3-13391]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Canine (100%), Feline (100%), Guinea Pig (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Cardiac Troponin T. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Cardiac Troponin T Antibody - BSA Free
![Western Blot: Cardiac Troponin T Antibody [NBP3-13391]](https://resources.bio-techne.com/images/products/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13391-img0006.jpg "Western Blot: Cardiac Troponin T Antibody [NBP3-13391]")
Western Blot: Cardiac Troponin T Antibody [NBP3-13391]
Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391]](https://resources.bio-techne.com/images/products/Cardiac-Troponin-T-Antibody-Immunohistochemistry-Paraffin-NBP3-13391-img0003.jpg "Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391]")
Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391]
Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391] - Rat heart. Cardiac Troponin T stained by Cardiac Troponin T antibody (NBP3-13391) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Western Blot: Cardiac Troponin T Antibody [NBP3-13391]](https://resources.bio-techne.com/images/products/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13391-img0001.jpg "Western Blot: Cardiac Troponin T Antibody [NBP3-13391]")
Western Blot: Cardiac Troponin T Antibody [NBP3-13391]
Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: Cardiac Troponin T Antibody [NBP3-13391]](https://resources.bio-techne.com/images/products/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13391-img0005.jpg "Western Blot: Cardiac Troponin T Antibody [NBP3-13391]")
![Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391]](https://resources.bio-techne.com/images/products/Cardiac-Troponin-T-Antibody-Immunohistochemistry-Paraffin-NBP3-13391-img0002.jpg "Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391]")
Applications for Cardiac Troponin T Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Troponin T
Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.
Alternate Names
CMH2, CMPD2, cTnT, TNNT2, TnTC
Gene Symbol
TNNT2
Additional Troponin T Products
Product Documents for Cardiac Troponin T Antibody - BSA Free
Product Specific Notices for Cardiac Troponin T Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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