Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44058

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0278

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Carbonic Anhydrase XII/CA12 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O43570, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Novus Biologicals Mouse Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free (NBP2-52901) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free

Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free Western Blot: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44058]

Western Blot: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44058]

Lane 1: Marker [kDa]
Lane 2: Human tonsil tissue lysate
Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44058]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44058]

Staining of human kidney shows strong membranous immunoreactivity in renal tubules, but not glomeruli.
Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44058]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP3-44058]

Staining of human renal cancer shows membranous positivity in tumor cells.
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Applications for Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XII/CA12

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Alternate Names

CA12

Gene Symbol

CA12

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Carbonic Anhydrase XII/CA12 Antibody (CL0278) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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