CAP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-58320
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to CAP1(CAP, adenylate cyclase-associated protein 1 (yeast)) The peptide sequence was selected from the middle region of CAP1.
Peptide sequence KAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQEN. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for CAP1 Antibody - BSA Free
Western Blot: CAP1 Antibody [NBP1-58320]
Western Blot: CAP1 Antibody [NBP1-58320] - THP-1 cell lysate, concentration 0.2-1 ug/ml.Immunohistochemistry: CAP1 Antibody [NBP1-58320]
Immunohistochemistry: CAP1 Antibody [NBP1-58320] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Plasma membrane Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 secWestern Blot: CAP1 Antibody - BSA Free [NBP1-58320] -
CAP1 is down‐regulated during terminal erythroid differentiation. A, A representative diagram of erythroid populations in E14.5 foetal livers, analysed by flow cytometry with antibodies against CD71 and Ter119. B‐D, R1 to R4 cell populations showed in (A) were sorted by flow cytometry. The mRNA levels of beta‐globin (B), pri‐miR‐144/451 (C) and Cap1 (D) were then determined by real‐time RT‐PCR assays. 18S (B & D) or U6 (C) rRNAs were used as the internal reference controls. E, Western blotting analyses of CAP1 protein levels in R1 to R4 cell populations. F, Gross view of the cell pellets from D0 and D2 in vitro differentiated foetal liver erythroid cells. G, D0 and D2 in vitro cultured foetal liver cells were stained with APC‐Ter119 and Hoechst 33342. Representative plots of flow cytometry were shown on the left and averaged percentage of Ter119high/Hoechstlow population from 3 independent experiments on the right. H, Relative expression levels of beta‐globin, pri‐miR‐144/451 and Cap1 from D0 and D2 in vitro cultured foetal liver cells. B‐D, E, G, H, All data are presented as mean +/- SEM from at least three biological replicates (*P < .05; **P < .01; ***P < .001) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33496386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for CAP1 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CAP1
Long Name
CAP, Adenylate Cyclase-Associated Protein 1
Alternate Names
CAP1-PEN
Gene Symbol
CAP1
UniProt
Additional CAP1 Products
Product Documents for CAP1 Antibody - BSA Free
Product Specific Notices for CAP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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