cAMP Antibody (RM466)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18822
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM466 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
KLH-conjugated cAMP
Specificity
This antibody reacts to cAMP. No cross reactivity with other cyclic nucleotide or nucleoside phosphate.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit cAMP Antibody (RM466) (NBP3-18822) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for cAMP Antibody (RM466)
ELISA: cAMP Antibody (RM466)[NBP3-18822]
ELISA: cAMP Antibody (RM466)[NBP3-18822]Competitive ELISA of cAMP using anti-cAMP (RM466). The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cAMP(RM466) (50 µl/well) was added and incubated. After wash and block, different concentrations of cAMP (25 µl/well) were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cAMP. (71-1358-00) TMB was used to develop the color after incubation and wash.
ELISA: cAMP Antibody (RM466) [NBP3-18822] -
ELISA: cAMP Antibody (RM466) [NBP3-18822] - Competitive ELISA detecting cAMP in HeLa cells. The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of RM466 (50 µl/well) was added and incubated. After wash and block, HeLa cell lysate (~105cell/mL) or control (lysate buffer only) (25 µl/well) were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cAMP.TMB was used to develop the color after incubation and wash.
ELISA: cAMP Antibody (RM466) [NBP3-18822] -
ELISA: cAMP Antibody (RM466) [NBP3-18822] - Competitive ELISA showing the specificity of RM466. The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of RM466 (50 µl/well) was added and incubated. After wash and block, cAMP and other cyclic nucleotides or nucleosidephosphates (25 µl/well) were added along with 25 µl/well of 1/5,000diluted HRP conjugated cAMP (71-1358-00). TMB was used to develop thecolor after incubation and wash.Applications for cAMP Antibody (RM466)
Application
Recommended Usage
ELISA
0.05 - 0.5 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: cAMP
cAMP (adenosine 3’5’-cyclic monophosphate) is a ubiquitous intracellular second messenger that mediates a diverse range of cellular processes in all organisms from bacteria to higher eukaryotes. It is converted from adenosine triphosphate (ATP) by adenylyl cyclases (ACs), and is inactivated by phosphodiesterases (PDEs) which catalyze its hydrolysis to 5’-AMP. In mammals, upon interaction with extracellular ligands, G protein coupled receptors (GPCRs) linked to Gαs activate the family of nine transmembrane ACs to increase intracellular cAMP. In contrast, GPCRs associated with Gαi/o inhibit the synthesis of cAMP by transmembrane ACs. cAMP binds and activates protein kinase A (PKA), the guanine nucleotide exchange factors Epac1/2, and cyclic nucleotide-gated ion channels. It can be released extracellularly where it is converted to adenosine by phosphodiesterases and nucleotidases.
Long Name
Cyclic AMP
Alternate Names
Cyclic AMP
Additional cAMP Products
Product Documents for cAMP Antibody (RM466)
Product Specific Notices for cAMP Antibody (RM466)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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