BTF Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89924
![Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunohistochemistry-Paraffin-NBP1-89924-img0009.jpg "Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]")
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QKSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKSYKDDSKHKREQDHSRSSSSSASPSSPSSR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit BTF Antibody - BSA Free (NBP1-89924) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BTF Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89924-img0005.jpg "Immunocytochemistry/ Immunofluorescence: BTF Antibody [NBP1-89924]")
Immunocytochemistry/ Immunofluorescence: BTF Antibody [NBP1-89924]
Immunocytochemistry/Immunofluorescence: BTF Antibody [NBP1-89924] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunohistochemistry-Paraffin-NBP1-89924-img0008.jpg "Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]")
Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]
Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924] - Staining of human bone marrow, cerebral cortex, colon and kidney using Anti-BCLAF1 antibody NBP1-89924 (A) shows similar protein distribution across tissues to independent antibody NBP1-89923 (B).![Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunohistochemistry-Paraffin-NBP1-89924-img0015.jpg "Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]")
Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]
Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924] - Staining of human colon.![Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunohistochemistry-Paraffin-NBP1-89924-img0011.jpg "Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]")
![Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunohistochemistry-Paraffin-NBP1-89924-img0012.jpg "Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]")
![Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunohistochemistry-Paraffin-NBP1-89924-img0013.jpg "Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]")
![Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunohistochemistry-Paraffin-NBP1-89924-img0014.jpg "Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]")
![Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]](https://resources.bio-techne.com/images/products/BTF-Antibody-Immunohistochemistry-Paraffin-NBP1-89924-img0016.jpg "Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]")
![BTF Antibody - BSA Free Immunohistochemistry-Paraffin: BTF Antibody - BSA Free [NBP1-89924]](https://resources.bio-techne.com/images/products/nbp1-89924_rabbit-polyclonal-btf-antibody-135202513504718.jpg "Immunohistochemistry-Paraffin: BTF Antibody - BSA Free [NBP1-89924]")
![BTF Antibody - BSA Free Western Blot: BTF Antibody - BSA Free [NBP1-89924]](https://resources.bio-techne.com/images/products/nbp1-89924_rabbit-polyclonal-btf-antibody-135202517442124.jpg "Western Blot: BTF Antibody - BSA Free [NBP1-89924]")
Applications for BTF Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BTF
Alternate Names
bcl-2-associated transcription factor 1, BCL2-associated transcription factor 1, Btf, BTFKIAA0164bK211L9.1
Gene Symbol
BCLAF1
Additional BTF Products
Product Documents for BTF Antibody - BSA Free
Product Specific Notices for BTF Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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