BRD9 Antibody (BLR153J)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14730

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14730
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR153J

Concentration

1 mg/ml

View all BRD9 Antibodies »

Product Specifications

Immunogen

between 547 and 597 (C-term)

Clonality

Monoclonal

Host

Rabbit

Description

Novus Biologicals Rabbit BRD9 Antibody (BLR153J) (NBP3-14730) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BRD9 Antibody (BLR153J)

Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]

Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]

Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730] - Whole cell lysate (30 ug) from CT26, CH27, and TCMK-1 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiBRD9 recombinant monoclonal antibody [BLR153J] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.Lower Panel: Rabbit anti-Actin recombinant monoclonalantibody [BLR057F].
BRD9 Antibody (BLR153J)

Simple Western: BRD9 Antibody [NBP3-14730] -

Simple Western: BRD9 Antibody [NBP3-14730] - Detection of human BRD9 by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (NBP3-14730) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image internally validated.
BRD9 Antibody (BLR153J)

Simple Western: BRD9 Antibody [NBP3-14730] -

Simple Western: BRD9 Antibody [NBP3-14730] - Detection of mouse BRD9 by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (NBP3-14730) used at 1:50 and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image internally validated.
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Applications for BRD9 Antibody (BLR153J)

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

6 ul/1 mg lysate

Simple Western

1:10-1:250

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat [0.4 mg/mL], separated by Size, antibody dilution of 1:10, 1:50, 1:250, apparent MW was 90-92 kDa

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with , BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: BRD9

Long Name

Bromodomain Containing 9

Alternate Names

FLJ13441, LAVS3040, PRO9856

Gene Symbol

BRD9

Additional BRD9 Products

Product Documents for BRD9 Antibody (BLR153J)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRD9 Antibody (BLR153J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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