BPTF/FALZ Antibody

Western Blot: BPTF/FALZ Antibody [NB100-41418]

Western Blot: BPTF/FALZ Antibody [NB100-41418] - Detection of Human FALZ/BPTF by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody NB100-41418 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: BPTF/FALZ Antibody [NB100-41418]

Immunoprecipitation: BPTF/FALZ Antibody [NB100-41418] - Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody NB100-41418 used for IP at 6 ug per reaction. For blotting immunoprecipitated FALZ/BPTF, NB100-41418 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: BPTF/FALZ Antibody [NB100-41418] -

Western Blot: BPTF/FALZ Antibody [NB100-41418] - Identification of alternative BPTF species in human cancer cells.(A) Reactivity of anti-BPTF antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines & detected with independent antibodies. The findings suggest the occurrence of multiple BPTF-related protein species in human cancer cells. A representative western blot is shown. (B) Gel bands selected for BPTF Mass Spectrometry identification according to the localization of the BPTF signal detected by western blotting in MCF-7 cells. (Left, NP-40 lysis buffer, gel bands A1 & A2; Right, Laemmli buffer, B1 & B2). The detection of low molecular weight species upon direct cell lysis in Laemmli buffer strongly supports the notion that the findings do not result from artifactual proteolysis. (C) Sequence coverage of BPTF protein. Peptides identified by LC-MS/MS are highlighted in color. (D) BPTF peptide intensity (arbitrary units) calculated by MaxQuant in the gel bands A1, A2/B1, B2. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31498079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BPTF/FALZ Antibody [NB100-41418] -

Western Blot: BPTF/FALZ Antibody [NB100-41418] - Identification of alternative BPTF species in human cancer cells.(A) Reactivity of anti-BPTF antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines & detected with independent antibodies. The findings suggest the occurrence of multiple BPTF-related protein species in human cancer cells. A representative western blot is shown. (B) Gel bands selected for BPTF Mass Spectrometry identification according to the localization of the BPTF signal detected by western blotting in MCF-7 cells. (Left, NP-40 lysis buffer, gel bands A1 & A2; Right, Laemmli buffer, B1 & B2). The detection of low molecular weight species upon direct cell lysis in Laemmli buffer strongly supports the notion that the findings do not result from artifactual proteolysis. (C) Sequence coverage of BPTF protein. Peptides identified by LC-MS/MS are highlighted in color. (D) BPTF peptide intensity (arbitrary units) calculated by MaxQuant in the gel bands A1, A2/B1, B2. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31498079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.