BMP-15/GDF-9B Antibody (BMP15/4263)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07552
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # BMP15/4263
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recognizes sequence SAEVTASSSKHSGPENNQC on the C-terminus of human BMP-15/GDF-9B (Uniprot: O95972)
Localization
Cytoplasmic (secreted)
Specificity
BMP15, also known as GDF9B, plays a crucial role in the regulation of fertility. Clone BMP15/4263 can be used to detect BMP15 expression and biosynthesis in various carcinoma progression studies.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08876)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Applications for BMP-15/GDF-9B Antibody (BMP15/4263)
Application
Recommended Usage
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: BMP-15/GDF-9B
BMP-15 (Bone morphogenetic protein 15), also known as GDF-9B, is a TGF-b superfamily ligand that is expressed by oocytes throughout folliculogenesis and plays an important role in oocyte development. BMP-15 promotes the FSH-independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis. BMP-15 blocks the FSH-induced GC expression of FSH R and multiple steroidogenic molecules. BMP-15 exerts is effects through interactions with BMPR-IB/ALK6 and BMPR-II, and nonphosphorylated BMP-15 functions as a competitive antagonist at BMPR-IB. Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9.
Long Name
Bone Morphogenetic Protein 15
Alternate Names
BMP15, GDF-9B
Gene Symbol
BMP15
UniProt
Additional BMP-15/GDF-9B Products
Product Documents for BMP-15/GDF-9B Antibody (BMP15/4263)
Product Specific Notices for BMP-15/GDF-9B Antibody (BMP15/4263)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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