Bmf Antibody (JE33-57)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32085

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32085
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE33-57 expressed in HEK293

Concentration

1 mg/ml

View all BMF Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Bmf aa 1-184 / 184. (Uniprot: Q96LC9)

Localization

Acrosomal vesicle, cytosol, mitochondrial outer membrane, myosin complex, plasma membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Bmf Antibody (JE33-57) (NBP3-32085) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Bmf Antibody (JE33-57)

Bmf Antibody (JE33-57)

Western Blot: Bmf Antibody (JE33-57) [NBP3-32085]

Western Blot: Bmf Antibody (JE33-57) [NBP3-32085] - Western blot analysis of Bmf on different lysates with Rabbit anti-Bmf antibody (NBP3-32085) at 1/1,000 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Rat spleen tissue lysate
Lane 5: Mouse pancreas tissue lysate
Lane 6: Rat pancreas tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 21 kDa
Observed band size: 30 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32085) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Bmf Antibody (JE33-57)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMF

Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.

Long Name

Bcl-2 Modifying Factor

Alternate Names

Bcl2 modifying factor, bcl-2-modifying factor, FLJ00065

Gene Symbol

BMF

Additional BMF Products

Product Documents for Bmf Antibody (JE33-57)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bmf Antibody (JE33-57)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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