Blue Marker Antibody (6F4-F6) - BSA Free

Western Blot: Blue Marker Antibody (6F4-F6)BSA Free [NBP2-33376]

Western Blot: Blue Marker Antibody (6F4-F6) [NBP2-33376] - Analysis using the HRP conjugate of NBP2-33376. Precision Plus Dual Color molecular weight markers (1 and 2 ul) were separated by SDS-PAGE on a 4-14% gel and transferred to PVDF membrane. The membrane was incubated with HRP conjugated Blue Marker Antibody at 1 ug/ml for 30 min. After washing, the membrane was exposed to ECL and the blue markers detected with a 5 second exposure.

Western Blot: Blue Marker Antibody (6F4-F6)BSA Free [NBP2-33376]

Western Blot: Blue Marker Antibody (6F4-F6) [NBP2-33376] - The indicated amounts of the BioRad Precision Plus Dual Color and All Blue protein standards were separated by SDS-PAGE on a 4-15% gel, transferred to PVDF membrane. The membrane was blocked for 1 hour in 5% non-fat milk in TBST and then incubated with 1.0 ug/mL of Anti-blue antibody and a 1:10,000 dilution of Anti-mouse HRP in 1% non-fat milk TBST for 1 hour. After washing the membrane was processed for chemiluminescent detection.

Western Blot: Blue Marker Antibody (6F4-F6)BSA Free [NBP2-33376]

Western Blot: Blue Marker Antibody (6F4-F6) [NBP2-33376] - Highly specific to blue prestained markers. Does not cross react with whole cell proteins of different species. Whole cell lysate from various cell lines of human, mouse, rat and hamster origin were separated on a 4-15% gel by electrophoresis and transferred to PVDF. The membrane was stained with Amido black to show the presence of protein from all lysates. After blocking in 5% non-fat milk, the membrane was incubated with Anti-Blue at 1 ug/ml. After washing and incubation with Anti-mouse HRP the blot was incubated in ECL reagent and imaged. Note that only the blue molecular weight markers were detected with the Anti-Blue antibody demonstrating its high specificity and suitability for addition to your Western blot procedure.