BIRC6 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB300-264
![Western Blot: BIRC6 Antibody [NB300-264]](https://resources.bio-techne.com/images/products/BIRC6-Antibody-Western-Blot-NB300-264-img0012.jpg "Western Blot: BIRC6 Antibody [NB300-264]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide derived from a sequence between residue 4775 and the C-terminus (residue 4829) of human Baculoviral IAP repeat-containing protein 6 using the numbering given in Swiss-Prot entry Q9NR09 (GeneID 57448).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit BIRC6 Antibody - BSA Free (NB300-264) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-BIRC6 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BIRC6 Antibody - BSA Free
Western Blot: BIRC6 Antibody [NB300-264]
Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264]](https://resources.bio-techne.com/images/products/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB300-264-img0008.jpg "Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264]")
Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264]
Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).![Immunoprecipitation: BIRC6 Antibody [NB300-264]](https://resources.bio-techne.com/images/products/BIRC6-Antibody-Immunoprecipitation-NB300-264-img0013.jpg "Immunoprecipitation: BIRC6 Antibody [NB300-264]")
Immunoprecipitation: BIRC6 Antibody [NB300-264]
Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for BIRC6 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:2000-1:10000
Immunohistochemistry-Paraffin
1:2000-1:10000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:33815677) Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BIRC6
Long Name
Baculoviral IAP repeat-containing protein 6
Alternate Names
APOLLON, BRUCE, EC 2.3.2.27, KIAA1289
Entrez Gene IDs
57448 (Human)
Gene Symbol
BIRC6
UniProt
Additional BIRC6 Products
Product Documents for BIRC6 Antibody - BSA Free
Product Specific Notices for BIRC6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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