Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92640

Novus Biologicals, part of Bio-Techne
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NBP2-92640-0.02ml
NBP2-92640-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Beta Hydroxysteroid Dehydrogenase Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1). MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free (NBP2-92640) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free [NBP2-92640]

Immunocytochemistry/ Immunofluorescence: Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free [NBP2-92640]

Immunocytochemistry/Immunofluorescence: Beta Hydroxysteroid Dehydrogenase Antibody [NBP2-92640] - Analysis of A-549 cells using Beta Hydroxysteroid Dehydrogenase .
Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free [NBP2-92640] -

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free [NBP2-92640] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Beta Hydroxysteroid Dehydrogenase, using Beta Hydroxysteroid Dehydrogenase Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.5s.

Applications for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Beta Hydroxysteroid Dehydrogenase

The protein encoded by the Beta Hydroxysteroid Dehydrogenases gene is a bifunctional enzyme that catalyzes the oxidative conversion ofdelta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in thebiosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads.Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternativelyspliced transcript variants have been found for this gene. (provided by RefSeq)

Alternate Names

3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD II, delta 5-delta 4-isomerase type II, HSD3B, HSDB, HSDB3B, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2,3-beta-hydroxy-Delta(5)-steroid dehydrogenase, progesterone reductase, SDR11E2, short chain dehydrogenase/reductase family 11E, member 2,3-beta-hydroxy-5-ene steroid dehydrogenase

Gene Symbol

HSD3B2

Additional Beta Hydroxysteroid Dehydrogenase Products

Product Documents for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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