Beta Dystroglycan Antibody (P38G1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] -

Immunocytochemistry/ Immunofluorescence: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on coverslips stained with the chimeric verison of this antibody, NBP3-43570 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cell membrane/junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43570, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Flow Cytometry: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] -

Flow Cytometry: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] - MCF7 cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the chimeric version of this antibody, NBP3-43570 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Western Blot: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] -

Western Blot: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] - Human skeletal muscle tissue lysate (35µg protein in RIPA buffer) was resolved on an SDS-PAGE gel, and blots were probed with the chimeric verison of this antibody, NBP3-43570 at 0.1µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.