beta-Arrestin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1515

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NB100-1515
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all beta-Arrestin 2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HDHIPLPRPQS corresponding to internal region according to NP_004304.1, NP_945355.1.

Specificity

This antibody is expected to recognise both reported isoforms 1, 2, 3, 5 and 6 (NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1). No crossreactivity is expected with Arrestin beta 1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for beta-Arrestin 2 Antibody

Western Blot: beta-Arrestin 2 Antibody [NB100-1515]

Western Blot: beta-Arrestin 2 Antibody [NB100-1515]

Western Blot: beta-Arrestin 2 Antibody [NB100-1515] - Staining of A549 (A),and U251 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: beta-Arrestin 2 Antibody [NB100-1515]

Immunocytochemistry/ Immunofluorescence: beta-Arrestin 2 Antibody [NB100-1515]

Immunocytochemistry/Immunofluorescence: beta-Arrestin 2 Antibody [NB100-1515] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: beta-Arrestin 2 Antibody [NB100-1515]

Flow Cytometry: beta-Arrestin 2 Antibody [NB100-1515]

Flow Cytometry: beta-Arrestin 2 Antibody [NB100-1515] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for beta-Arrestin 2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.5 - 1 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Arrestin 2

Members of arrestin/beta arrestin protein family are thought to participate in agonist mediated desensitization of G protein coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors.

Alternate Names

ARB2, ARR2, ARRB2, betaArrestin 2

Entrez Gene IDs

409 (Human)

Gene Symbol

ARRB2

UniProt

NP_004304.1 (Human)

Additional beta-Arrestin 2 Products

Product Documents for beta-Arrestin 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for beta-Arrestin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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