Beta-Amylase Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-20638
Key Product Details
Species Reactivity
Plant
Applications
Western Blot, ELISA, Dot Blot
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Beta-Amylase [Sweet Potato] (Uniprot: P10537)
Reactivity Notes
Cross reactivity against Beta Amylase from other sources may occur but has not been specifically determined.
Localization
Cytoplasmic
Specificity
Cross reactivity against b Amylase from other sources may occur but has not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato]
Scientific Data Images for Beta-Amylase Antibody [Biotin]
Dot Blot: Beta-Amylase Antibody [Biotin] [NB120-20638] - Dot Blot results of Rabbit Beta-Amylase antibody [Biotin].Antigen: Beta-Amylase.Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng.Blocking Buffer for 30 minutes at RT.Primary Antibody: Rabbit Anti-Beta-Amylase Biotin 1ug/mL for 1hr at RT.Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30min at RT.Imaging System ChemiDoc, Filter used: Chemi.
Applications for Beta-Amylase Antibody [Biotin]
Application
Recommended Usage
ELISA
1:5000-1:25000
Western Blot
1:500-1:3000
Application Notes
This product has been tested by dot blot and western blot. This product is assayed against 1.0 ug of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Beta-Amylase
Alternate Names
14 alpha D glucan maltohydrolase, 1-4 alpha D glucan maltohydrolase, AMYB, BMY1
UniProt
Additional Beta-Amylase Products
Product Documents for Beta-Amylase Antibody [Biotin]
Product Specific Notices for Beta-Amylase Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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