beta-Actin Antibody (RM112)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18847
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM112 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A peptide corresponding to the N-terminus of beta-Actin (Uniprot # P60709)
Specificity
No cross reactivity with other isoforms of Actin.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit beta-Actin Antibody (RM112) (NBP3-18847) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for beta-Actin Antibody (RM112)
Western Blot: beta-Actin Antibody (RM112)[NBP3-18847]
Western Blot: beta-Actin Antibody (RM112)[NBP3-18847]Western blot of A431 cells, using anti-beta -Actin RevMAb Clone RM112 at a 1/1000 dilution, showed a band of beta-Actin at the predicted MW (42 kDa).
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (RM112) [NBP3-18847] -
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (RM112) [NBP3-18847] - Immunocytochemical staining of HeLa cells, using Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
Immunohistochemistry-Paraffin: beta-Actin Antibody (RM112) [NBP3-18847] -
Immunohistochemistry-Paraffin: beta-Actin Antibody (RM112) [NBP3-18847] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue using Clone RM112 at a 1:1000 dilution.Applications for beta-Actin Antibody (RM112)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:200
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry
1:200
Immunoprecipitation
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: beta-Actin
References
1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245
2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619
3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968
4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433
Alternate Names
ACTB, betaActin
Gene Symbol
ACTB
Additional beta-Actin Products
Product Documents for beta-Actin Antibody (RM112)
Product Specific Notices for beta-Actin Antibody (RM112)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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