BCL2L12 Antibody (4Z2Z8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15930
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4Z2Z8 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human BCL2L12 (Q9HB09). GPPSTEKEAILRRLVALLEEEAEVINQKLASDPALRSKLVRLSSDSFARLVELFCSRDDSSRPSRACPGPPPPSPEPLARLALAMELSRRVAGLGGTLAGL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit BCL2L12 Antibody (4Z2Z8) (NBP3-15930) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BCL2L12 Antibody (4Z2Z8)
![Western Blot: BCL2L12 Antibody (4Z2Z8) [NBP3-15930]](https://resources.bio-techne.com/images/products/BCL2L12-Antibody-4Z2Z8-Western-Blot-NBP3-15930-img0002.jpg "Western Blot: BCL2L12 Antibody (4Z2Z8) [NBP3-15930]")
Western Blot: BCL2L12 Antibody (4Z2Z8) [NBP3-15930]
Western Blot: BCL2L12 Antibody (4Z2Z8) [NBP3-15930] - Western blot analysis of extracts of various cell lines, using BCL2L12 Rabbit mAb (NBP3-15930) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.![Immunohistochemistry-Paraffin: BCL2L12 Antibody (4Z2Z8) [NBP3-15930]](https://resources.bio-techne.com/images/products/BCL2L12-Antibody-4Z2Z8-Immunohistochemistry-Paraffin-NBP3-15930-img0001.jpg "Immunohistochemistry-Paraffin: BCL2L12 Antibody (4Z2Z8) [NBP3-15930]")
Immunohistochemistry-Paraffin: BCL2L12 Antibody (4Z2Z8) [NBP3-15930]
Immunohistochemistry-Paraffin: BCL2L12 Antibody (4Z2Z8) [NBP3-15930] - Immunohistochemistry of paraffin-embedded human esophageal cancer using BCL2L12 Rabbit mAb (NBP3-15930) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation: BCL2L12 Antibody (4Z2Z8) [BCL2L12] -
Immunoprecipitation: BCL2L12 Antibody (4Z2Z8) [BCL2L12] - Immunoprecipitation analysis of 300 ug extracts of PC-3 cells using 3 ug BCL2L12 antibody . Western blot was performed from the immunoprecipitate using BCL2L12 antibody at a dilution of 1:500.Applications for BCL2L12 Antibody (4Z2Z8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:100 - 1:500
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BCL2L12
Alternate Names
Bcl-2 related proline-rich protein, bcl2-L-12, BCL2-like 12 (proline rich), bcl-2-like protein 12, Bcl-2-related proline-rich protein, BPR, MGC120313, MGC120314, MGC120315
Gene Symbol
BCL2L12
Additional BCL2L12 Products
Product Documents for BCL2L12 Antibody (4Z2Z8)
Product Specific Notices for BCL2L12 Antibody (4Z2Z8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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