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Baboon Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-664

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NB300-664-0.05mg
NB300-664-0.025mg
NB300-664

Key Product Details

Species Reactivity

Drosophila

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide: CNKNENMKRIFETDYVPHRLTS, corresponding to N terminal amino acids 200-221 of Drosophilia melanogaster Baboon. The peptide was amidated for conjugation.

Reactivity Notes

Fruit fly.

Specificity

The is unique to Baboon protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Baboon Antibody

Western Blot: Baboon Antibody [NB300-664]

Western Blot: Baboon Antibody [NB300-664]

Western Blot: Baboon Antibody [NB300-664] - Lane 1 and 2 : Cells expressing Baboon A. Lane 3: mock trasfected cells

Applications for Baboon Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Immunocytochemistry, Immunoprecipitation, Western Blot Detects a band of approximately 73 kDa. Optimal dilutions and concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Baboon

Transmembrane receptor Patched regulates several developmental processes in both invertebrate and vertebrates. In higher vertebrates the patched (Ptc) also acts as tumor suppressor. The patched receptor signaling involves negatively regulating a GPCR, smoothened. The binding Hedgehog ligand to Ptc, removes the negative interaction of Ptc and allows the signaling by smoothened. It has been shown that Ptc D. melanogasater head development. During head morphogenesis in D. melanogaster Ptc positively interacts with smoothened, which leads to stimulation of Activin type 1 receptor Baboon and activation of cell proliferation in the eye-antennal disc (3). The expression of activated Baboon in the Ptc domain in Ptc mutant background completely abolish the head developmental defects. Baboon is a 601 amino acid long (Mr. Wt. 73 kDa) protein with a single transmembrane domain. FabGennix International Inc. has produced epitope specific anti-Baboon antibodies in rabbit. The Anti-Baboon-selective antibodies were generated against a peptide sequence near the C-terminal end of the protein that are unique to Baboon protein. The Baboon-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The Baboon antibodies label a 73-74 kDa protein in D. melanogaster extracts. Anti-Baboon-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.

Alternate Names

Activin A receptor 45A, ATR 1, Atr 45A, Atr I, ATR1, Atr45A, AtrI, Bab, Babo, CG8224, G protein coupled receptor kinase 3, Gprk 3, Gprk3, l(2)k16912, RE55648p, STK E, STKE

Additional Baboon Products

Product Documents for Baboon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Baboon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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