B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09018
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # HK5.3
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizing SD rat with mouse B7h-transfected L cells and fusing immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells.
Specificity
This antibody specifically binds to mouse ICOSL and competes with binding of ICOS.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]](https://resources.bio-techne.com/images/products/B7-H2-ICOSLG-Antibody-HK5-3-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09018-img0002.jpg "Immunocytochemistry/ Immunofluorescence: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]")
Immunocytochemistry/ Immunofluorescence: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]
Immunocytochemistry/Immunofluorescence: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric [NBP3-09018] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of HK5.3 (NBP3-09018) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing weak membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09018, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.![ELISA: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]](https://resources.bio-techne.com/images/products/B7-H2-ICOSLG-Antibody-HK5-3-Chimeric-ELISA-NBP3-09018-img0001.jpg "ELISA: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]")
ELISA: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free [NBP3-09018]
ELISA: B7-H2/ICOSLG Antibody (HK5.3) - Chimeric [NBP3-09018] - ELISA Plate coated with B7-H2/ICOSLG-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP3-09018. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used.Applications for B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
This antibody has been used to detect murine ICOSL by flow cytometry. It is able to block ICOSL interacting with ICOSL and inhibit T-cell proliferation in a collagen type II (CII)-induced arthritis model.
This antibody has been used to detect murine ICOSL by flow cytometry. It is able to block ICOSL interacting with ICOSL and inhibit T-cell proliferation in a collagen type II (CII)-induced arthritis model.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: B7-H2
Long Name
B7 Homolog 2
Alternate Names
B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG
Gene Symbol
ICOSLG
Additional B7-H2 Products
Product Documents for B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free
Product Specific Notices for B7-H2/ICOSLG Antibody (HK5.3) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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