B7-1/CD80 Antibody (62N3G8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25255
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - BSA Free [NBP2-25255]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-62N3G8-Flow-Cytometry-NBP2-25255-img0009.jpg "Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - BSA Free [NBP2-25255]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, COMET
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 62N3G8
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Amino acids 102-223 of human CD80 were used as the immunogen for the antibody.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 27440778).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for B7-1/CD80 Antibody (62N3G8) - BSA Free
Detection of CD80/B7-1 in Human Hodgkin’s Lymphoma via seqIF™ staining on COMET™
CD80/B7-1 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Mouse Anti-Human CD80/B7-1, Monoclonal Antibody (Catalog #NBP2-25255) at 20ug/mL at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.![Western Blot: B7-1/CD80 Antibody (62N3G8)BSA Free [NBP2-25255]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-62N3G8-Western-Blot-NBP2-25255-img0010.jpg "Western Blot: B7-1/CD80 Antibody (62N3G8)BSA Free [NBP2-25255]")
Western Blot: B7-1/CD80 Antibody (62N3G8)BSA Free [NBP2-25255]
Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) - BSA Free [NBP2-25255]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-62N3G8-Immunohistochemistry-Paraffin-NBP2-25255-img0002.jpg "Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) - BSA Free [NBP2-25255]")
Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) - BSA Free [NBP2-25255]
Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.![Western Blot: B7-1/CD80 Antibody (62N3G8)BSA Free [NBP2-25255]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-62N3G8-Western-Blot-NBP2-25255-img0003.jpg "Western Blot: B7-1/CD80 Antibody (62N3G8)BSA Free [NBP2-25255]")
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - BSA Free [NBP2-25255]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-62N3G8-Flow-Cytometry-NBP2-25255-img0008.jpg "Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - BSA Free [NBP2-25255]")
Applications for B7-1/CD80 Antibody (62N3G8) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000. Use reported by customer review
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Multiplex Immunofluorescence
20ug/mL
Western Blot
reported by customer review
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Reviewed Applications
Read 2 reviews rated 5 using NBP2-25255 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: B7-1/CD80
Additional B7-1/CD80 Products
Product Documents for B7-1/CD80 Antibody (62N3G8) - BSA Free
Product Specific Notices for B7-1/CD80 Antibody (62N3G8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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